IMAGING CELL BIOLOGY IN LIVING SYSTEMS
The focus of this new course will be to study relevant problems in cell biology, immunology, developmental biology and neurobiology and how they have been solved using imaging approaches. For example at the cell level we will investigate how techniques such as TIRF and high speed confocal have addressed basic problems in endocytosis; at the organismal level we will use multiphoton, confocal, fret, and other approaches to understand aspects of cell biology in cell polarity, respiration and organ development in c. Elegans, Drosophila, Zebra fish and mice. In each case the application will focus on how imaging tools are used to study defined problems in living systems. The course will follow a lecture/demo/journal club format. Lectures will be two part, the first 1/3 will be a description of the technology, how it was developed and how it works (10-15 minutes) followed by description of the scientific problem and how it was solved. This will be followed by lab demonstrations showing the approach in action. Lectures will be interspersed with a journal club discussion of a relevant paper on each technology. Students will prepare the journal club presentations in an alternating fashion. Examination will be a combination of class participation, journal club and written exam.